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human pancreatic primary islets  (Prodo Labs)
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pancreatic islets  (Insphero Inc)
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Evaluation of HLA mismatch impact on co-culture sensitivity and background activation. (A) Liver spheroid co-cultures comparing autologous and allogeneic (HLA-A*02 matched) effector donors. Data are presented as mean ± SD as shaded area of technical replicates (n=3) from 3 representative donor pairs (N = 3) for each HLA-A*02 background. Statistical significance relative of the autologous cultures compared to corresponding allogenic cultures two-way ANOVA with Tukey’s post-hoc test, p values are indicated for the overall comparison between autologous versus allogeneic data. (B) <t>Pancreatic</t> islet spheroid co-cultures comparing HLA-matched (4/6 alleles) and HLA-mismatched conditions. Data illustrate the increased signal-to-noise ratio in HLA-matched systems compared to the elevated background activation observed in allogeneic co-cultures. Data are presented as mean of N = 5 donors for the matched dataset and N = 4 donors for the unmatched dataset run in technical replicates (n=2).
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human pancreatic islets  (Prodo Labs)
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Evaluation of HLA mismatch impact on co-culture sensitivity and background activation. (A) Liver spheroid co-cultures comparing autologous and allogeneic (HLA-A*02 matched) effector donors. Data are presented as mean ± SD as shaded area of technical replicates (n=3) from 3 representative donor pairs (N = 3) for each HLA-A*02 background. Statistical significance relative of the autologous cultures compared to corresponding allogenic cultures two-way ANOVA with Tukey’s post-hoc test, p values are indicated for the overall comparison between autologous versus allogeneic data. (B) <t>Pancreatic</t> islet spheroid co-cultures comparing HLA-matched (4/6 alleles) and HLA-mismatched conditions. Data illustrate the increased signal-to-noise ratio in HLA-matched systems compared to the elevated background activation observed in allogeneic co-cultures. Data are presented as mean of N = 5 donors for the matched dataset and N = 4 donors for the unmatched dataset run in technical replicates (n=2).
Human Pancreatic Islets, supplied by Prodo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evaluation of HLA mismatch impact on co-culture sensitivity and background activation. (A) Liver spheroid co-cultures comparing autologous and allogeneic (HLA-A*02 matched) effector donors. Data are presented as mean ± SD as shaded area of technical replicates (n=3) from 3 representative donor pairs (N = 3) for each HLA-A*02 background. Statistical significance relative of the autologous cultures compared to corresponding allogenic cultures two-way ANOVA with Tukey’s post-hoc test, p values are indicated for the overall comparison between autologous versus allogeneic data. (B) <t>Pancreatic</t> islet spheroid co-cultures comparing HLA-matched (4/6 alleles) and HLA-mismatched conditions. Data illustrate the increased signal-to-noise ratio in HLA-matched systems compared to the elevated background activation observed in allogeneic co-cultures. Data are presented as mean of N = 5 donors for the matched dataset and N = 4 donors for the unmatched dataset run in technical replicates (n=2).
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pancreatic islet cells  (Diabetology)
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Evaluation of HLA mismatch impact on co-culture sensitivity and background activation. (A) Liver spheroid co-cultures comparing autologous and allogeneic (HLA-A*02 matched) effector donors. Data are presented as mean ± SD as shaded area of technical replicates (n=3) from 3 representative donor pairs (N = 3) for each HLA-A*02 background. Statistical significance relative of the autologous cultures compared to corresponding allogenic cultures two-way ANOVA with Tukey’s post-hoc test, p values are indicated for the overall comparison between autologous versus allogeneic data. (B) <t>Pancreatic</t> islet spheroid co-cultures comparing HLA-matched (4/6 alleles) and HLA-mismatched conditions. Data illustrate the increased signal-to-noise ratio in HLA-matched systems compared to the elevated background activation observed in allogeneic co-cultures. Data are presented as mean of N = 5 donors for the matched dataset and N = 4 donors for the unmatched dataset run in technical replicates (n=2).
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baron human 26 pancreatic islets indrop  (10X Genomics)
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Evaluation of HLA mismatch impact on co-culture sensitivity and background activation. (A) Liver spheroid co-cultures comparing autologous and allogeneic (HLA-A*02 matched) effector donors. Data are presented as mean ± SD as shaded area of technical replicates (n=3) from 3 representative donor pairs (N = 3) for each HLA-A*02 background. Statistical significance relative of the autologous cultures compared to corresponding allogenic cultures two-way ANOVA with Tukey’s post-hoc test, p values are indicated for the overall comparison between autologous versus allogeneic data. (B) <t>Pancreatic</t> islet spheroid co-cultures comparing HLA-matched (4/6 alleles) and HLA-mismatched conditions. Data illustrate the increased signal-to-noise ratio in HLA-matched systems compared to the elevated background activation observed in allogeneic co-cultures. Data are presented as mean of N = 5 donors for the matched dataset and N = 4 donors for the unmatched dataset run in technical replicates (n=2).
Baron Human 26 Pancreatic Islets Indrop, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse pancreatic islet β cell line nit 1  (ATCC)
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Evaluation of HLA mismatch impact on co-culture sensitivity and background activation. (A) Liver spheroid co-cultures comparing autologous and allogeneic (HLA-A*02 matched) effector donors. Data are presented as mean ± SD as shaded area of technical replicates (n=3) from 3 representative donor pairs (N = 3) for each HLA-A*02 background. Statistical significance relative of the autologous cultures compared to corresponding allogenic cultures two-way ANOVA with Tukey’s post-hoc test, p values are indicated for the overall comparison between autologous versus allogeneic data. (B) <t>Pancreatic</t> islet spheroid co-cultures comparing HLA-matched (4/6 alleles) and HLA-mismatched conditions. Data illustrate the increased signal-to-noise ratio in HLA-matched systems compared to the elevated background activation observed in allogeneic co-cultures. Data are presented as mean of N = 5 donors for the matched dataset and N = 4 donors for the unmatched dataset run in technical replicates (n=2).
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mouse pancreatic islet b cell line nit  (ATCC)
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Evaluation of HLA mismatch impact on co-culture sensitivity and background activation. (A) Liver spheroid co-cultures comparing autologous and allogeneic (HLA-A*02 matched) effector donors. Data are presented as mean ± SD as shaded area of technical replicates (n=3) from 3 representative donor pairs (N = 3) for each HLA-A*02 background. Statistical significance relative of the autologous cultures compared to corresponding allogenic cultures two-way ANOVA with Tukey’s post-hoc test, p values are indicated for the overall comparison between autologous versus allogeneic data. (B) <t>Pancreatic</t> islet spheroid co-cultures comparing HLA-matched (4/6 alleles) and HLA-mismatched conditions. Data illustrate the increased signal-to-noise ratio in HLA-matched systems compared to the elevated background activation observed in allogeneic co-cultures. Data are presented as mean of N = 5 donors for the matched dataset and N = 4 donors for the unmatched dataset run in technical replicates (n=2).
Mouse Pancreatic Islet B Cell Line Nit, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse pancreatic islets  (Jackson Laboratory)
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Jackson Laboratory mouse pancreatic islets
Evaluation of HLA mismatch impact on co-culture sensitivity and background activation. (A) Liver spheroid co-cultures comparing autologous and allogeneic (HLA-A*02 matched) effector donors. Data are presented as mean ± SD as shaded area of technical replicates (n=3) from 3 representative donor pairs (N = 3) for each HLA-A*02 background. Statistical significance relative of the autologous cultures compared to corresponding allogenic cultures two-way ANOVA with Tukey’s post-hoc test, p values are indicated for the overall comparison between autologous versus allogeneic data. (B) <t>Pancreatic</t> islet spheroid co-cultures comparing HLA-matched (4/6 alleles) and HLA-mismatched conditions. Data illustrate the increased signal-to-noise ratio in HLA-matched systems compared to the elevated background activation observed in allogeneic co-cultures. Data are presented as mean of N = 5 donors for the matched dataset and N = 4 donors for the unmatched dataset run in technical replicates (n=2).
Mouse Pancreatic Islets, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evaluation of HLA mismatch impact on co-culture sensitivity and background activation. (A) Liver spheroid co-cultures comparing autologous and allogeneic (HLA-A*02 matched) effector donors. Data are presented as mean ± SD as shaded area of technical replicates (n=3) from 3 representative donor pairs (N = 3) for each HLA-A*02 background. Statistical significance relative of the autologous cultures compared to corresponding allogenic cultures two-way ANOVA with Tukey’s post-hoc test, p values are indicated for the overall comparison between autologous versus allogeneic data. (B) Pancreatic islet spheroid co-cultures comparing HLA-matched (4/6 alleles) and HLA-mismatched conditions. Data illustrate the increased signal-to-noise ratio in HLA-matched systems compared to the elevated background activation observed in allogeneic co-cultures. Data are presented as mean of N = 5 donors for the matched dataset and N = 4 donors for the unmatched dataset run in technical replicates (n=2).

Journal: Frontiers in Immunology

Article Title: Translational strategy to support the first-in-human study of a TCR-like T cell bispecific with an in vitro -based safety approach

doi: 10.3389/fimmu.2026.1736584

Figure Lengend Snippet: Evaluation of HLA mismatch impact on co-culture sensitivity and background activation. (A) Liver spheroid co-cultures comparing autologous and allogeneic (HLA-A*02 matched) effector donors. Data are presented as mean ± SD as shaded area of technical replicates (n=3) from 3 representative donor pairs (N = 3) for each HLA-A*02 background. Statistical significance relative of the autologous cultures compared to corresponding allogenic cultures two-way ANOVA with Tukey’s post-hoc test, p values are indicated for the overall comparison between autologous versus allogeneic data. (B) Pancreatic islet spheroid co-cultures comparing HLA-matched (4/6 alleles) and HLA-mismatched conditions. Data illustrate the increased signal-to-noise ratio in HLA-matched systems compared to the elevated background activation observed in allogeneic co-cultures. Data are presented as mean of N = 5 donors for the matched dataset and N = 4 donors for the unmatched dataset run in technical replicates (n=2).

Article Snippet: Pancreatic Islets: 3D InSight Islet Microtissues (Cat: MT-04-002-01-60, InSphero) composed of primary alpha, beta, and delta cells were used.

Techniques: Co-Culture Assay, Activation Assay, Comparison